Rheumatoid arthritis (RA) is a chronic disease characterized by the presence of inflammatory events and triggered immunity. Oxidative stress has been linked to the deterioration of many pathological conditions and involved in the presence of serious health risks. Reactive oxygen species (ROS) is involved in beneficial and harmful effects to the biological system. When the levels of ROS are increased the harmful effects appeared in different levels of oxidative damage. The oxidative damage of the lipids is termed lipid peroxidation and can be screened by measuring one of the final products called malondialdehyde (MDA). Antioxidants are the materials that responsible for the detoxification of ROS and prevent their harmful effects. In this article, we aimed to determine the levels of MDA, total oxidant status (TOS), and total antioxidant capacity (TAC) in patients with RA disease. Furthermore, we goaled to investigate the relationship of oxidative stress with RA disease, and the possibility of using oxidative stress indicators as diagnostic markers for the disease. The results have shown increase in the oxidation state of RA patients. The levels of MDA and TOS were significantly elevated in the serum of RA patients, in addition to this, the level of TAC was significantly reduced. This shift in the redox balance towered the oxidants indicate for sure the development of oxidative stress in RA patients. Furthermore, MDA, TOS, and TAC were observed to be excellent sensitive biomarkers for the diagnosis of RA disease. From this results, the administration with antioxidants is very important in the treatment of RA patients to control the disease and prevent any associated risks.