This research aimed to determine the effect of Whey protein on the biochemical levels of reproductive hormones in male albino rats. An adult male albino rats (n= 36) were randomly divided into three groups (12 in each group). First and second groups orally administrated with (0.8 and 1.6 g) of whey protein, respectively, and third group as control administrated with normal saline for sixty days. After administration, body weights were recorded, the blood and seminal fluid were collected for physiological and fertility studies. The results showed a significant increase (p<0.05) in the mean body weights of treated group compared with control group. Hormones level showed treated groups were a significantly increased (p≤0.05) in the mean of testosterone concentration, a significant decrease (p≤0.05) in the mean of prolactin concentration and the luteinizing hormone concentration, but there is no significant change (p≤0.05) has been noticed in the mean of follicle stimulating hormone concentration, compared to the control group. The sperms concentration rate (X107 ) increased significantly (p≤ 0.05), also, sperm characteristics showed the motility of sperms increased significantly in the percentage rate (p≤ 0.05), whereas the dead and abnormal sperms decreased significantly (p≤ 0.05) in the percentage rate. Regarding the fertility for the current study revealed there was no significant increasing (p<0.05) in the number of births within treated groups compared with control group.